Hi C

Hi C. Thus HiC is the method of choice for the chromosomescale scaffolding of genome assemblies Please see for example this paper “Scaffolding of long read assemblies using long range contact information” by Ghurye et al The HiC libraries are sequenced on Illumina sequencers At the moment we offer HiC for animal samples.

OverviewHistoryExperimental methodsBiological impactData analysisFurther readingSee alsoChromosome conformation capture techniques (often abbreviated to 3C technologies or 3Cbased methods ) are a set of molecular biology methods used to analyze the spatial organization of chromatin in a cell These methods quantify the number of interactions between genomic loci that are nearby in 3D space but may be separated by many nucleotidesin the linear genome Such in Text under.

Bypass

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HiC: A Method to Study the Threedimensional …

HiC is the first of the 3C derivative technologies to be truly genomewide After the restriction digest biotinlabeled nucleotides are annealed to the fragment end overhangs The blunt ends of the DNA fragments are ligated A biotin pulldown then isolates only ligated fragments The library is then sequenced and mapped to the genome allowing fragment identity and abundance to be.

Orange Lavaburst HiC®

HiC data analysis is a process involving multiple steps that can be separated in preprocessing ie from raw data to the HiC contact matrix and downstream analyses (Ay et al 2014 Schmitt et al 2016b) Preprocessing starts with FASTQ files of pairedend reads obtained from highthroughput sequencing that are (1) first of all aligned to the reference genome (2).